Alu PV92 PCR Lab
Purpose: The purpose of this lab was to find the length of our alu gene (genotype) in order to discover where our ancestors were from. This also led us to learning if we are homozygous +, homozygous -, or heterozygous.
For materials and procedure refer to http://babec.org/files/PCR_2012/Alu_PCR_Student_Guide_2012.pdf
Results: My results for this lab were that I discovered that my genotype is heterozygous. My DNA was in the fourth lane from the left in the picture below.
Purpose: The purpose of this lab was to find the length of our alu gene (genotype) in order to discover where our ancestors were from. This also led us to learning if we are homozygous +, homozygous -, or heterozygous.
For materials and procedure refer to http://babec.org/files/PCR_2012/Alu_PCR_Student_Guide_2012.pdf
Results: My results for this lab were that I discovered that my genotype is heterozygous. My DNA was in the fourth lane from the left in the picture below.
Analysis: I was able to figure out that my genotype was heterozygous because there were two pink bands in the square of gel after it went through gel electrophoresis. Since I am heterozygous, that means that one or both of my parents must be as well, or one of my parents is homozygous + and the other is homozygous -.
Conclusion: We didn't have any errors during our lab so we successfully found our genotypes which was our purpose of the lab. The one error I was concerned that we might have made was pipetting the wrong amounts because sometimes some of the liquid would get stuck in the tip but we ended up completing the lab with no errors. What we did in this lab was isolate DNA from our cheek cells and then we used PCR to determine the genotype of our alu locus. Finally, we used gel electrophoresis to analyze the results.
Conclusion: We didn't have any errors during our lab so we successfully found our genotypes which was our purpose of the lab. The one error I was concerned that we might have made was pipetting the wrong amounts because sometimes some of the liquid would get stuck in the tip but we ended up completing the lab with no errors. What we did in this lab was isolate DNA from our cheek cells and then we used PCR to determine the genotype of our alu locus. Finally, we used gel electrophoresis to analyze the results.